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KMID : 0616120180490040312
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Korean Journal of Pharmacognosy
2018 Volume.49 No. 4 p.312 ~ p.321
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Simultaneous Analysis of the Compounds of Natural Cosmetic Resources Containing Chrysanthemum zawadskii, Perilla frutescens, Rosa multiflora and their Anti-oxidative Activity
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Ham Ha-Neul
Shrestha Abinash Chandra
Kim Ju-Eun
Lee Tae-Bum
Yoo Byoung-Wan
Kim Min-Sook
Kim Kwang-Sang
Cha Joon-Seok
Lee Yong-Mun
Kim Jong-Yeob
Leem Jae-Yoon
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Abstract
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Recently, consumer demand for functional cosmetics containing natural ingredients has been greatly expanded. To develop the natural cosmetic materials, we selected 3 plants, Chrysanthemum zawadskii Herbich (CZ), Perilla frutescens (L.) Britton var. acuta Kudo (PF), and Rosa multiflora Thunberg (RM) which showed high total flavonoid contents (TFC), total polyphenol contents (TPC), and strong DPPH radical scavenging effect. We determined astragalin, chlorogenic acid, and rosmarinic acid as a marker compound for quantitative analysis of the content of each material and standardization of the quality standards and manufacturing standards through LC/MS analysis. HPLC-DAD was used to simultaneously analyze these marker components of three natural product complexes (Mix) and to validate the analytical method through experiments such as linearity, accuracy and precision. The detection wavelengths were set at 210, 265, and 330 nm. The detected 3 compounds from extract of CZ, PF, RM showed significant linearity (R2=0.9947). The limit of detection (LOD) of chlorogenic acid, astragalin and rosmarinic acid were 8.29 μg/ml, 2.28 μg/ml, and 27.00 μg/ml, respectively. The limit of quantification (LOQ) of chlorogenic acid, astragalin and rosmarinic acid were 25.11 μg/ml, 6.92 μg/ml, and 81.83 μg/ml, respectively. The contents of the three indicators of Mix were 19.82-24.71 mg/g of chlorogenic acid, 43.80-46.02 mg/g of astragalin, and 46.33-48.57 mg/g of rosmarinic acid.
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KEYWORD
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Polyphenol contents, Flavonoid contents, Marker compound, Anti-oxidants, Simultaneous analysis, Natural cosmetics
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